Question:

4. Post-transformation culture plates After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X

4. Post-transformation culture plates
After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X-gal, and the
antibiotic spectinomycin.
PART 1:
1. What would have happened if we had cultured bacteria from any of the two starter plates in the
post-transformation culture plates?
2. Why was the addition of the antibiotic spectinomycin important in the port-transformation
cultures?

4. Post-transformation culture plates After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X-gal, and the antibiotic spectinomycin. PART 1: 1. What would have happened if we had cultured bacteria from any of the two starter plates in the post-transformation culture plates? 2. Why was the addition of the antibiotic spectinomycin important in the port-transformation cultures?

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