4. Post-transformation culture plates
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Question

4. Post-transformation culture plates
After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X-gal, and the
antibiotic spectinomycin.
PART 1:
1. What would have happened if we had cultured bacteria from any of the two starter plates in the
post-transformation culture plates?
2. Why was the addition of the antibiotic spectinomycin important in the port-transformation
cultures?

Kunduz · Accepted Answer

we
two starter
transformation
Answer -
1. When
cultured bacteria from any of the
starter plantes in the post
Culture pleetes,
plates Stopped
Growth of bacterice of Starter
Antibiotic
Streptomycin important in the post-
transformation cultures because streptomycin
growth of Non- toonsformed bacteria and
used for drug Senotivity test for transformed
bacteria.
le
stops
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4. Post-transformation culture plates After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X